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2c0y
From Proteopedia
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THE CRYSTAL STRUCTURE OF A CYS25ALA MUTANT OF HUMAN PROCATHEPSIN S
Overview
The crystal structure of the active-site mutant Cys25 --> Ala of, glycosylated human procathepsin S is reported. It was determined by, molecular replacement and refined to 2.1 Angstrom resolution, with an, R-factor of 0.198. The overall structure is very similar to other, cathepsin L-like zymogens of the C1A clan. The peptidase unit comprises, two globular domains, and a small third domain is formed by the N-terminal, part of the prosequence. It is anchored to the prosegment binding loop of, the enzyme. Prosegment residues beyond the prodomain dock to the substrate, binding cleft in a nonproductive orientation. Structural comparison with, published data for mature cathepsin S revealed that procathepsin S, residues Phe146, Phe70, and Phe211 adopt different orientations. Being, part of ... [(full description)]
About this Structure
2C0Y is a [Single protein] structure of sequence from [Homo sapiens]. Active as [Cathepsin S], with EC number [3.4.22.27]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].
Reference
The crystal structure of a Cys25 -> Ala mutant of human procathepsin S elucidates enzyme-prosequence interactions., Kaulmann G, Palm GJ, Schilling K, Hilgenfeld R, Wiederanders B, Protein Sci. 2006 Nov;15(11):2619-29. PMID:17075137
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