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Introduction

Isocitrate lyase is a lyase found in the proteome of multiple bacteria that oxidizes the hydroxl group of isocitrate and cleaves the substrate in two forming glyoxylate and succinate. Isocitrate lyase is a tetramer that is composed primarily of alpha helices and beta sheets with a unique structural phenomenon called "". This enzyme can be found within the cytosol of bacteria and is used in a variation of the citric acid cycle to help conserve energy by not using NADPH as an electron carrier and by reforming coenzyme-A earlier than in the normal citric acid cycle.

Isocitrate Lyase

Structure

Figure 1. Crystal Structure of Isocitrate Lyase. Quaternary structure is comprised of four subunits forming an alpha/beta barrel.

Isocitrate lyase is a tetramer with 222 symmetry. Each subunit is composed of 14 alpha helices and 14 beta sheets which includes a total of 426 residues. These alpha helices and beta sheets form an unusual alpha/beta barrel seen in Figure 1.

Helix Swapping

A unique structural feature of this enzyme is a phenomenon called "". Helix swapping is observed between two monomers to form stable dimers. The 11th and 12th helices of each monomer exchange three dimensional placement with the respective helices of the opposite monomer. Due to the 222 symmetry observed, only two dimers form than combine to form the observed tetramer. As a result of this structure, 18% of the surface of each monomer is buried within the protein.

Active Site Residues

Figure 2. Active Site Loop Shift. Binding of the ligand to the enzyme results in a conformational shift that facilitates the breakdown of isocitrate. The active site loop unbound is shown in wheat and the active site loop bound is shown in green.

Figure 3. Active site residues hydrogen bound to a cofactor and the products of the catalyzed isocitrate reaction. Glyoxylate is shown in blue, succinate is shown in green, and the Mg²⁺ cofactor is shown in yellow.

Figure 4. Active Site Residues. All eight active site residues necessary for catalysis of isocitrate are shown in slate. However, the protein shown is a C191S mutant of isocitrate lyase.

Inhibitors

Regulation

Isocitrate lyase competes with [isocitrate dehydrogenase], an enzyme found in the [citric cycle], for isocitrate processing. Flux through these enzymes is controlled by phosphorylation of isocitrate dehydrogenase, which has a much higher affinity for isocitrate as compared to ICL.[6] Deactivation of isocitrate dehydrogenase by phosphorylation thus leads to increased isocitrate channeling through ICL, as seen when bacteria are grown on acetate, a two-carbon compound.

Mechanism of Action

Disease Association

Clinical Implications

Mycobacterium tuberculosis is a respiratory infection that causes numerous fatalities throughout the world. It lives in organisms and feeds off of host cells, which indicate a variety of lipases exist within M. tuberculosis. Current drugs that are on the market now target a small number of bacterial processes like cell wall formation and chromosomal replication. Although several antibiotics exist, all of them target these same mechanisms of inhibition. These commonalities have led to the prevalence of different multi-drug resistant (MDR) tuberculosis strains. Due to the high level of resistance, finding a lasting treatment for MDR TB infections has become very problematic. Studies into new mechanisms of inhibition will be crucial to prevent widespread outbreaks. Isocitrate lyase plays a key role in survival of M. tuberculosis by sustaining intracellular infections in inflammatory respiratory macrophages. Used in the citric acid cycle, isocitrate lyase is the first enzyme catalyzing the carbon conserving glyoxylate pathway. This glyoxylate pathway has not been observed in mammals and thus presents a unique drug target to solely attack TB infections.