3bts
From Proteopedia
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Crystal structure of a ternary complex of the transcriptional repressor Gal80p (Gal80S0 [G301R]) and the acidic activation domain of Gal4p (aa 854-874) from Saccharomyces cerevisiae with NAD
Overview
Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UAS(GAL)); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5'-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.
About this Structure
3BTS is a Protein complex structure of sequences from Saccharomyces cerevisiae with as ligand. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
NADP regulates the yeast GAL induction system., Kumar PR, Yu Y, Sternglanz R, Johnston SA, Joshua-Tor L, Science. 2008 Feb 22;319(5866):1090-2. PMID:18292341
Page seeded by OCA on Wed Mar 5 13:24:06 2008
Categories: Protein complex | Saccharomyces cerevisiae | Joshua-Tor, L. | Kumar, P R. | NAD | Acetylation | Activator | Carbohydrate metabolism | Dna-binding | Eukaryotic transcription complex | Galactose metabolism | Metal-binding | Nad | Nucleus | Phosphoprotein | Repressor | Rossmann fold | Transcription regulation | Zinc
