Structural highlights
4ws1 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , , |
| Related: | 4wru, 4wrv, 4wrw, 4wrx, 4wry, 4wrz, 4ws0, 4ws2, 4ws3, 4ws4, 4ws5, 4ws6, 4ws7, 4ws8 |
| Activity: | Uracil-DNA glycosylase, with EC number 3.2.2.27 |
| Resources: | FirstGlance, OCA, RCSB, PDBsum |
Function
[UNG_MYCTU] Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine (By similarity).
Publication Abstract from PubMed
Uracil N-glycosylase (Ung) is the most thoroughly studied of the group of uracil DNA-glycosylase (UDG) enzymes that catalyse the first step in the uracil excision-repair pathway. The overall structure of the enzyme from Mycobacterium tuberculosis is essentially the same as that of the enzyme from other sources. However, differences exist in the N- and C-terminal stretches and some catalytic loops. Comparison with appropriate structures indicate that the two-domain enzyme closes slightly when binding to DNA, while it opens slightly when binding to the proteinaceous inhibitor Ugi. The structural changes in the catalytic loops on complexation reflect the special features of their structure in the mycobacterial protein. A comparative analysis of available sequences of the enzyme from different sources indicates high conservation of amino-acid residues in the catalytic loops. The uracil-binding pocket in the structure is occupied by a citrate ion. The interactions of the citrate ion with the protein mimic those of uracil, in addition to providing insights into other possible interactions that inhibitors could be involved in.
Structure of uracil-DNA glycosylase from Mycobacterium tuberculosis: insights into interactions with ligands.,Kaushal PS, Talawar RK, Varshney U, Vijayan M Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Aug 1;66(Pt, 8):887-92. Epub 2010 Jul 27. PMID:20693660[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kaushal PS, Talawar RK, Varshney U, Vijayan M. Structure of uracil-DNA glycosylase from Mycobacterium tuberculosis: insights into interactions with ligands. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Aug 1;66(Pt, 8):887-92. Epub 2010 Jul 27. PMID:20693660 doi:10.1107/S1744309110023043
