1hk8
From Proteopedia
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| , resolution 2.45Å | |||||||
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| Sites: | |||||||
| Ligands: | , and | ||||||
| Activity: | Ribonucleoside-triphosphate reductase, with EC number 1.17.4.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURAL BASIS FOR ALLOSTERIC SUBSTRATE SPECIFICITY REGULATION IN CLASS III RIBONUCLEOTIDE REDUCTASES: NRDD IN COMPLEX WITH DGTP
Overview
A Zn(Cys)(4) center has been found in the C-terminal region of the crystal structure of the anaerobic class III ribonucleotide reductase (RNR) from bacteriophage T4. The metal center is structurally related to the zinc ribbon motif and to rubredoxin and rubrerythrin. Mutant enzymes of the homologous RNR from Escherichia coli, in which the coordinating cysteines, conserved in almost all known class III RNR sequences, have been mutated into alanines, are shown to be inactive as the result of their inability to generate the catalytically essential glycyl radical. The possible roles of the metal center are discussed in relationship to the currently proposed reaction mechanism for generation of the glycyl radical in class III RNRs.
About this Structure
1HK8 is a Single protein structure of sequence from Enterobacteria phage t2. This structure supersedes the now removed PDB entry 1H77. Full crystallographic information is available from OCA.
Reference
A metal-binding site in the catalytic subunit of anaerobic ribonucleotide reductase., Logan DT, Mulliez E, Larsson KM, Bodevin S, Atta M, Garnaud PE, Sjoberg BM, Fontecave M, Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3826-31. Epub 2003 Mar 24. PMID:12655046
Page seeded by OCA on Thu Mar 20 11:38:11 2008
