2eu8

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spinqualitylabelsall models PDB ID 2eu8 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
, resolution 1.800Å
Ligands:	, , and
Gene:	adk (Bacillus subtilis)
Activity:	Adenylate kinase, with EC number 2.7.4.3
Coordinates:	save as pdb, mmCIF, xml

Crystal structure of a thermostable mutant of Bacillus subtilis Adenylate Kinase (Q199R)

Overview

In nature, evolution occurs through the continuous adaptation of a population to its environment. At the molecular level, adaptive changes in protein sequence and expression impact organismal fitness and, consequently, dictate population dynamics. Here, we have used a "weak link" method to favor variations in one gene, allowing adaptation to thermostability to be studied in molecular detail as bacteria were grown continuously for approximately 1500 generations. Surprisingly, only six mutant alleles, representing less than 1% of the possible missense mutations, were observed, suggesting a highly constrained molecular landscape during protein evolution. The changes in organismal fitness were linked directly to incremental increases in enzyme stability and activity maxima and corresponded to the narrow temperature ranges where each mutant enjoyed success within the overall population. Thus, continuous evolution of a single gene permits a quantitative approach that extends from the phenotypes of the microbial populations to their underlying biophysical basis.

About this Structure

2EU8 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

In vivo molecular evolution reveals biophysical origins of organismal fitness., Counago R, Chen S, Shamoo Y, Mol Cell. 2006 May 19;22(4):441-9. PMID:16713575

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