2k50

Structural highlights

Function

[O27438_METTH] Inhibits DNA polymerase activity of PolB, which can be overcome by RFC and PNCA. Stimulates 3'-to 5'-exonuclease activity of PolB at 30 degrees Celsius, but has no effect at 50 or 70 degrees Celsius. Bind ssDNA and replication forks; replication forks structures bind both Hel308 and this protein. Has no effect on helicase activity of Hel308; may help target the helicase to DNA substrates that require DNA re-modeling.^{[1] [2]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

1. ↑ Kelman Z, Pietrokovski S, Hurwitz J. Isolation and characterization of a split B-type DNA polymerase from the archaeon Methanobacterium thermoautotrophicum deltaH. J Biol Chem. 1999 Oct 1;274(40):28751-61. PMID:10497247
2. ↑ Woodman IL, Brammer K, Bolt EL. Physical interaction between archaeal DNA repair helicase Hel308 and Replication Protein A (RPA). DNA Repair (Amst). 2011 Mar 7;10(3):306-13. doi: 10.1016/j.dnarep.2010.12.001., Epub 2010 Dec 30. PMID:21195035 doi:http://dx.doi.org/10.1016/j.dnarep.2010.12.001