Structural highlights
1tlw is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | |
| Related: | 1tly, 1tlz |
| Gene: | TSX, NUPA, B0411, Z0512, ECS0464, SF0348, S0356 (Escherichia coli) |
| Resources: | FirstGlance, OCA, RCSB, PDBsum |
Function
[TSX_ECOLI] Constitutes the receptor for colicin K and phage T6, and functions as substrate-specific channel for nucleosides and deoxynucleosides.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Tsx is a nucleoside-specific outer membrane (OM) transporter of Gram-negative bacteria. We present crystal structures of Escherichia coli Tsx in the absence and presence of nucleosides. These structures provide a mechanism for nucleoside transport across the bacterial OM. Tsx forms a monomeric, 12-stranded beta-barrel with a long and narrow channel spanning the outer membrane. The channel, which is shaped like a keyhole, contains several distinct nucleoside-binding sites, two of which are well defined. The base moiety of the nucleoside is located in the narrow part of the keyhole, while the sugar occupies the wider opening. Pairs of aromatic residues and flanking ionizable residues are involved in nucleoside binding. Nucleoside transport presumably occurs by diffusion from one binding site to the next.
Crystal structure of the bacterial nucleoside transporter Tsx.,Ye J, van den Berg B EMBO J. 2004 Aug 18;23(16):3187-95. Epub 2004 Jul 22. PMID:15272310[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ye J, van den Berg B. Crystal structure of the bacterial nucleoside transporter Tsx. EMBO J. 2004 Aug 18;23(16):3187-95. Epub 2004 Jul 22. PMID:15272310 doi:10.1038/sj.emboj.7600330
