1juy
From Proteopedia
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REFINED CRYSTAL STRUCTURE OF ADENYLOSUCCINATE SYNTHETASE FROM ESCHERICHIA COLI COMPLEXED WITH HYDANTOCIDIN 5'-PHOSPHATE GDP, HPO4(2-), MG2+, AND HADACIDIN
Overview
A crystal structure of adenylosuccinate synthetase from Escherichia coli, complexed with 5'-phosphate, GDP, HPO4(2-), Mg2+, and hadacidin at 100 K, has been refined to an Rfactor of 0.195 against data to 2.6 A resolution., Bond lengths and angles deviate from expected values by 0.012 A and 1.86, degrees, respectively. Lys 16 and backbone amides 15-17 and 42 interact, with the phosphates of GDP, while Ser 414, Asp 333, and backbone amides, 331 and 416 interact with the base. Mg2+ is octahedrally coordinated., Oxygen atoms from GDP, phosphate, and hadacidin define the equatorial, plane of coordination of the Mg2+, while backbone carbonyl 40 and the side, chain of Asp 13 are the apical ligands. HPO4(2-) hydrogen bonds with Lys, 16, His 41, backbone amides 13, 40, and 224, and the base moiety ... [(full description)]
About this Structure
1JUY is a [Single protein] structure of sequence from [Escherichia coli] with MG, PI, GDP, H5P and HDA as [ligands]. Active as [Adenylosuccinate synthase], with EC number [6.3.4.4]. Structure known Active Sites: ASP, GNS and IMP. Full crystallographic information is available from [OCA].
Reference
Refined crystal structure of adenylosuccinate synthetase from Escherichia coli complexed with hydantocidin 5'-phosphate, GDP, HPO4(2-), Mg2+, and hadacidin., Poland BW, Lee SF, Subramanian MV, Siehl DL, Anderson RJ, Fromm HJ, Honzatko RB, Biochemistry. 1996 Dec 10;35(49):15753-9. PMID:8961938
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