1f59
From Proteopedia
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| , resolution 2.8Å | |||||||
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IMPORTIN-BETA-FXFG NUCLEOPORIN COMPLEX
Overview
We describe the crystal structure of a complex between importin-beta residues 1-442 (Ib442) and five FxFG nucleoporin repeats from Nsp1p. Nucleoporin FxFG cores bind on the convex face of Ib442 to a primary site between the A helices of HEAT repeats 5 and 6, and to a secondary site between HEAT repeats 6 and 7. Mutations at importin-beta Ile178 in the primary FxFG binding site reduce both binding and nuclear protein import, providing direct evidence for the functional significance of the importin-beta-FxFG interaction. The FxFG binding sites on importin-beta do not overlap with the RanGTP binding site. Instead, RanGTP may release importin-beta from FxFG nucleoporins by generating a conformational change that alters the structure of the FxFG binding site.
About this Structure
1F59 is a Single protein structure of sequence from Homo sapiens and Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structural basis for the interaction between FxFG nucleoporin repeats and importin-beta in nuclear trafficking., Bayliss R, Littlewood T, Stewart M, Cell. 2000 Jul 7;102(1):99-108. PMID:10929717
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