From Proteopedia

proteopedia linkproteopedia link

This Sandbox is Reserved from January 19, 2016, through August 31, 2016 for use for Proteopedia Team Projects by the class Chemistry 423 Biochemistry for Chemists taught by Lynmarie K Thompson at University of Massachusetts Amherst, USA. This reservation includes Sandbox Reserved 425 through Sandbox Reserved 439.

Contents 1 Penicillin binding protein/lactivicin (inhibitor) (2jch)[1] 2 Introduction 3 =Overall Structure 3.1 Binding Interactions 3.2 Additional Features 3.3 Quiz Question 1 3.4 See Also 3.5 Credits 3.6 References

Penicillin binding protein/lactivicin (inhibitor) (2jch)^[1]

by Tyler Carpenter, Samuel Pierce, Hyunjoon Choi, Anton El Khoury and Tiankai Zhang

Student Projects for UMass Chemistry 423 Spring 2016

Introduction

spinqualitylabelsall models 2jch, Saves cells from dying since the appearance of life Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Outline: 1) Why is it important? Penicillin-binding proteins are essential for cell wall biogenesis. General info about bacterial cell wall biogenesis. Also, how bacteria mutate in order to avoid binding to beta-lactam. 2) Ligands, in particular . Also, how PBP binds lactivicin. Sample green scene: Penicillin-binding protein has four ligands, one of which is

=Overall Structure

The secondary structures of PBP have certain affects on ligands Ligand has oxyanion hole to further binding potential. How does this help?

spinqualitylabelsall models 2jch, insert caption here Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Binding Interactions

spinqualitylabelsall models 2jch, Insert caption here Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Additional Features

1)role in antibiotics and resistance the role of PBPs in its synthesis is a very good target for drugs of selective toxicity

2)different PBPs and how they are classified into diverse categories found as both membrane-bound and cytoplasmic proteins

3)work as enzyme to catalyze synthesis of peptidoglycan

spinqualitylabelsall models 2jch, Insert caption here Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Quiz Question 1

Question related to PBP evolution and developing resistance to ß-lactams, likely related to active site mutations. Shows the from most conserved to most variable.

spinqualitylabelsall models 2jch, Insert caption here Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

See Also

• Penicillin-binding protein
• 2y2g
• 2bg1
• 2fff
• 2jch

Credits

Introduction - Anton El Khoury

Overall Structure - Tyler Carpenter

Drug Binding Site - name of team member

Additional Features - Tiankai Zhang

Quiz Question 1 - Samuel Pierce

References

1. ↑ Macheboeuf P, Fischer DS, Brown T Jr, Zervosen A, Luxen A, Joris B, Dessen A, Schofield CJ. Structural and mechanistic basis of penicillin-binding protein inhibition by lactivicins. Nat Chem Biol. 2007 Sep;3(9):565-9. Epub 2007 Aug 5. PMID:17676039 doi:10.1038/nchembio.2007.21