Introduction
The intercalation of DNA and drug compounds has been studied thoroughly, in this case the nucleotide d(CGTACG) was complexed with an anthraquinone derivative. This derivative, 1,5-bis[3-(diethylamino)propionamido]anthracene-9,10-dione, provided researchers with the information needed to solve using X-Ray crystallography. Along with the structure, the important forces involved in binding were analyzed and described as heavily reliant on cations. Furthermore, the binding site seems to be specific to anthracene and similar molecules. Therefore, the potential for drug compounds to be carrying by this nucleotide complex requires further research.
Everyone -- note that this is NOT a protein, but a complex with DNA. Edit your text accordingly, eg discussing alpha helices etc does not make sense.
Overall Structure
The 1xcs (model at right) complex is a small, simple globular DNA-peptide complex, and as such lacks secondary beta sheets or alpha helices. The complex consists of two non-identical dimers. A simplified model of 1xcs is shown with the side chains removed for clarity. The peptide backbones can be followed from N to C termini following along each strand from blue to red. Note that the strands are antiparallel where they are non-covalently bound. visualizes this bonding in the middle region of the complex, again following each strand from blue to red from N to C terminus.
Binding Interactions
Additional Features
Click to see that the drug binds directly between strands of DNA. This interrupts the expression of this section of DNA.
I will then go on to explain how specifically this complex interrupts/changes gene expression.
Quiz Question 1
Why is it important for the protein to bind ?
A unique site is the __ ion found associated with the , which gives additional strength to the guanine–Co2+–guanine interaction. A Mg2+ B Co2+ C Ba2+ D Mg2+
See Also
Credits
Introduction - Daniel Marco
Overall Structure - Nathaneal Park
Drug Binding Site - Annie Burton
Additional Features - Michael Beauregard
Quiz Question 1 - Jianlong Li
References
- ↑ Valls N, Steiner RA, Wright G, Murshudov GN, Subirana JA. Variable role of ions in two drug intercalation complexes of DNA. J Biol Inorg Chem. 2005 Aug;10(5):476-82. Epub 2005 Sep 23. PMID:15926069 doi:10.1007/s00775-005-0655-3
