2ca6

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spinqualitylabelsall models 2ca6, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

MIRAS STRUCTURE DETERMINATION FROM HEMIHEDRALLY TWINNED CRYSTALS

Overview

The structure of Rna1p was originally solved to 2.7 A resolution by MIRAS, from crystals with partial hemihedral twinning in space group I4(1), [Hillig et al. (1999), Mol. Cell, 3, 781-791] by finding a low-twinned, native crystal (twin fraction alpha=0.06) and after twin correction of all, data sets. Rna1p crystals have now been used to examine how far twinning, and twin correction affect MIR phasing with a higher resolution but highly, twinned native data set. Even high hemihedral twinning [alphanative=0.39, alphaderivative=0.24] would not have hindered heavy-atom site, identification of strong derivatives using difference Patterson maps., However, a weaker derivative could have been missed and refinement would, have stalled at high R values had twinning not been identified and, ... [(full description)]

About this Structure

2CA6 is a [Single protein] structure of sequence from [Schizosaccharomyces pombe] with SO4 as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Detecting and overcoming hemihedral twinning during the MIR structure determination of Rna1p., Hillig RC, Renault L, Acta Crystallogr D Biol Crystallogr. 2006 Jul;62(Pt 7):750-65. Epub 2006, Jun 20. PMID:16790931

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