NAD(P) transhydrogenase (PTH) catalyzes the conversion of NADP and NADH to NADPH and NAD. See NAD and NAD(P)H. The reaction is coupled with proton translocation across the cell membrane while the enzyme undergoes conformational change[1]. PTH uses FAD as cofactor.
Structural highlights
PTH is composed of 3 domains:
- Domain I binds NAD(+)/NADH.
- Domain II is a membrane-spanning domain.
- Domain III binds NADP(+)/NADPH.
PTH is composed of 2 subunits. Subunit β contains domain III and part of II. The active site of PTH contains NAD in domain I and NADP in domain III[2].
