Penicillin acylase

From Proteopedia

proteopedia linkproteopedia link

Template:STRUCTURE 1fxv

Contents 1 Function 2 Relevance 3 Structural highlights 4 3D structures of penicillin acylase 5 References

Function

Penicillin acylase (PAH) catalyzes the conversion of penicillin to 6-amino-penicillanate and phenylacetate (PA). PAH contains 2 non-identical subunits. The larger β subunit contains a phenylmethylsulfonyl residue which is required for enzymatic activity. PAH participates in penicillin and cephalosporin biosynthesis^[1].

Relevance

PAH is used commercially for production of semi-synthetic penicillins.

Structural highlights

PAH contains a β-lactam binding site between its 2 subunits^[2].

3D structures of penicillin acylase

Updated on 19-June-2016

References

1. ↑ Duggleby HJ, Tolley SP, Hill CP, Dodson EJ, Dodson G, Moody PC. Penicillin acylase has a single-amino-acid catalytic centre. Nature. 1995 Jan 19;373(6511):264-8. PMID:7816145 doi:http://dx.doi.org/10.1038/373264a0
2. ↑ Alkema WB, Hensgens CM, Kroezinga EH, de Vries E, Floris R, van der Laan JM, Dijkstra BW, Janssen DB. Characterization of the beta-lactam binding site of penicillin acylase of Escherichia coli by structural and site-directed mutagenesis studies. Protein Eng. 2000 Dec;13(12):857-63. PMID:11239085