Structural highlights
Publication Abstract from PubMed
In this study, crystals of Z-DNA hexamer d(CGCGCG) complexed with MgCl2 and CaCl2 were obtained in the presence of high concentrations of alkaline earth salts (500mM) using a temperature control technique, and their crystal structures were determined at 1.3A resolution. Mg2+ and Ca2+ cations in these structures tend to interact directly with phosphate groups of Z-DNA duplexes; however, they tend to form water-mediated interactions with Z-DNA in the presence of lower concentrations of alkaline earth salts. In these crystals, a DNA duplex was laid along its c-axis and interacted with its 6 neighboring DNA duplexes through coordination bonds of PO...(Mg2+ or Ca2+)...OP. A symmetrical hexagonal Z-DNA duplex assembly model may explain DNA condensation caused by alkaline earth salts. These structures offer insights into the functions of alkaline earth cations essential to the structures and assembly of Z-DNA duplexes.
Direct interactions between Z-DNA and alkaline earth cations, discovered in the presence of high concentrations of MgCl and CaCl,Chatake T, Sunami T J Inorg Biochem. 2013 Mar 19;124C:15-25. doi: 10.1016/j.jinorgbio.2013.03.004. PMID:23578858[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chatake T, Sunami T. Direct interactions between Z-DNA and alkaline earth cations, discovered in the presence of high concentrations of MgCl and CaCl J Inorg Biochem. 2013 Mar 19;124C:15-25. doi: 10.1016/j.jinorgbio.2013.03.004. PMID:23578858 doi:10.1016/j.jinorgbio.2013.03.004
