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1m0g

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Revision as of 10:37, 20 March 2008 by OCA (Talk | contribs)
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PDB ID 1m0g

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Solution structure of the alpha domain of mt_nc


Overview

The structure of [113Cd(7)]-metallothionein (MT_nc) of the Antarctic fish Notothenia coriiceps, the first three-dimensional structure of a fish metallothionein, was determined by homonuclear 1H NMR experiments and heteronuclear [1H, 113Cd]-correlation spectroscopy. MT_nc is composed of an N-terminal beta domain with 9 cysteines and 3 metal ions and a carboxy-terminal alpha-domain with 11 cysteines and 4 metal ions. The position of the ninth Cys of the alpha domain of MT_nc is different from the corresponding Cys of mammalian MTs. As a result, the last CXCC motif in the mammalian MT sequence becomes CXXXCC in the fish MT. This difference leads to a structural change of the alpha domain and, in turn, to a different charge distribution with respect to that observed in mammalian metallothioneins.

About this Structure

1M0G is a Single protein structure of sequence from Notothenia coriiceps. Full crystallographic information is available from OCA.

Reference

Solution structure of MT_nc, a novel metallothionein from the Antarctic fish Notothenia coriiceps., Capasso C, Carginale V, Crescenzi O, Di Maro D, Parisi E, Spadaccini R, Temussi PA, Structure. 2003 Apr;11(4):435-43. PMID:12679021

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