1ma8
From Proteopedia
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| , resolution 1.3Å | |||||||
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A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine
Overview
Selenium was incorporated into an oligodeoxynucleotide in the form of 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 A, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2'-alpha-position with selenium does not affect the preference of the sugar for the C3'-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidines.
About this Structure
1MA8 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle. Multiwavelength anomalous dispersion., Teplova M, Wilds CJ, Wawrzak Z, Tereshko V, Du Q, Carrasco N, Huang Z, Egli M, Biochimie. 2002 Sep;84(9):849-58. PMID:12458077
Page seeded by OCA on Thu Mar 20 12:40:57 2008
