1mgx

From Proteopedia

proteopedia linkproteopedia link

spinqualitylabelsall models PDB ID 1mgx Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
Activity:	Coagulation factor IXa, with EC number 3.4.21.22
Coordinates:	save as pdb, mmCIF, xml

COAGULATION FACTOR, MG(II), NMR, 7 STRUCTURES (BACKBONE ATOMS ONLY)

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

The blood coagulation and regulatory proteins that contain gamma-carboxyglutamic acid are a part of a unique class of membrane binding proteins that require calcium for their interaction with cell membranes. Following protein biosynthesis, glutamic acids on these proteins are converted to gamma-carboxyglutamic acid (Gla) in a reaction that requires vitamin K as a cofactor. The vitamin K-dependent proteins undergo a conformational transition upon metal ion binding, but only calcium ions mediate protein-phospholipid interaction. To identify the site on Factor IX that is required for phospholipid binding, we have determined the three-dimensional structure of the Factor IX Gla domain bound to magnesium ions by NMR spectroscopy. By comparison of this structure to that of the Gla domain bound to calcium ions, we localize the membrane binding site to a highly ordered structure including residues 1-11 of the Gla domain. In the presence of Ca2+, Factor IX Gla domain peptides that contain the photoactivatable amino acid p-benzoyl-L-phenylalanine at positions 6 or 9 cross-link to phospholipid following irradiation, while peptides lacking this amino acid analog or with this analog at position 46 did not cross-link. These results indicate that the NH2 terminus of the Gla domain, specifically including leucine 6 and phenylalanine 9 in the hydrophobic patch, is the contact surface on Factor IX that interacts with the phospholipid bilayer.

Disease

Known diseases associated with this structure: Hemophilia B OMIM:[306900], Warfarin sensitivity OMIM:[306900]

About this Structure

1MGX is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Identification of the phospholipid binding site in the vitamin K-dependent blood coagulation protein factor IX., Freedman SJ, Blostein MD, Baleja JD, Jacobs M, Furie BC, Furie B, J Biol Chem. 1996 Jul 5;271(27):16227-36. PMID:8663165

Page seeded by OCA on Thu Mar 20 12:43:18 2008