Structural highlights
Function
[RADA_METVO] Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules (By similarity).
Publication Abstract from PubMed
RAD51 mediates homologous recombination (HR) by forming an active DNA nucleoprotein filament (NPF). A conserved aspartate that forms a salt bridge with the ATP gamma-phosphate is found at the nucleotide-binding interface between RAD51 subunits of the NPF known as the ATP cap. The salt bridge accounts for the nonphysiological cation(s) required to fully activate the RAD51 NPF. In contrast, RecA homologs and most RAD51 paralogs contain a conserved lysine at the analogous structural position. We demonstrate that substitution of human RAD51(D316) with lysine [HsRAD51(D316K)] decreases NPF turnover and facilitates considerably improved recombinase functions. Structural analysis shows that archaebacterial Methanococcus voltae RadA(D302K) [MvRAD51(D302K)] and HsRAD51(D316K) form extended active NPFs without salt. These studies suggest that the HsRAD51(D316) salt bridge may function as a conformational sensor that enhances turnover at the expense of recombinase activity.
The RAD51 ATP cap regulates nucleoprotein filament stability.,Amunugama R, He Y, Willcox S, Forties RA, Shim KS, Bundschuh R, Luo Y, Griffith J, Fishel R J Biol Chem. 2012 Jan 24. PMID:22275364[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Amunugama R, He Y, Willcox S, Forties RA, Shim KS, Bundschuh R, Luo Y, Griffith J, Fishel R. The RAD51 ATP cap regulates nucleoprotein filament stability. J Biol Chem. 2012 Jan 24. PMID:22275364 doi:10.1074/jbc.M111.239426
