Structural highlights
Publication Abstract from PubMed
Despite impressive advances in theories, methods and technologies, crystallization still remains a serious bottleneck in structural determination of macromolecules. Here we present a novel solid-liquid interface method (SLIM) for protein crystallization, based on the pre-adding and drying of a crystallization reagent, and thereafter the dispensing of a protein solution to the dried media to initiate crystallization from the solid-liquid interface. Not only quick and easy to perform, the method also allows for a less concentrated protein solution for setting up crystallization trials.
Solid-liquid interface method (SLIM): a new crystallization method for proteins.,Brostromer E, Nan J, Li LF, Su XD Biochem Biophys Res Commun. 2009 Sep 4;386(4):634-8. doi:, 10.1016/j.bbrc.2009.06.086. Epub 2009 Jun 21. PMID:19545537[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Brostromer E, Nan J, Li LF, Su XD. Solid-liquid interface method (SLIM): a new crystallization method for proteins. Biochem Biophys Res Commun. 2009 Sep 4;386(4):634-8. doi:, 10.1016/j.bbrc.2009.06.086. Epub 2009 Jun 21. PMID:19545537 doi:http://dx.doi.org/10.1016/j.bbrc.2009.06.086
