1nvp
From Proteopedia
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| , resolution 2.10Å | |||||||
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| Gene: | TBP OR TFIID OR TF2D (Homo sapiens), GTF2A1 OR TF2A1 (Homo sapiens), GTF2A2 OR TF2A2 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HUMAN TFIIA/TBP/DNA COMPLEX
Contents |
Overview
RNA polymerase II-dependent transcription requires the assembly of a multi-protein, preinitiation complex on core promoter elements. Transcription factor IID (TFIID) comprising the TATA box-binding protein (TBP) and TBP-associated factors (TAFs) is responsible for promoter recognition in this complex. Subsequent association of TFIIA and TFIIB provides enhanced complex stability. TFIIA is required for transcriptional stimulation by certain viral and cellular activators, and favors formation of the preinitiation complex in the presence of repressor NC2. The X-ray structures of human and yeast TBP/TFIIA/DNA complexes at 2.1A and 1.9A resolution, respectively, are presented here and seen to resemble each other closely. The interactions made by human TFIIA with TBP and DNA within and upstream of the TATA box, including those involving water molecules, are described and compared to the yeast structure. Of particular interest is a previously unobserved region of TFIIA that extends the binding interface with TBP in the yeast, but not in the human complex, and that further elucidates biochemical and genetic results.
Disease
Known diseases associated with this structure: Huntington disease-like-4 OMIM:[600075], Parkinson disease OMIM:[600075], Spinocerebellar ataxia 17 OMIM:[600075]
About this Structure
1NVP is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Novel interactions between the components of human and yeast TFIIA/TBP/DNA complexes., Bleichenbacher M, Tan S, Richmond TJ, J Mol Biol. 2003 Sep 26;332(4):783-93. PMID:12972251
Page seeded by OCA on Thu Mar 20 13:02:17 2008
