Structural highlights
Function
[MYH10_HUMAN] Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension; this function is mechanically antagonized by MYH9.[1] [2]
Publication Abstract from PubMed
We determined the crystal structure of the motor domain of human non-muscle myosin 2B (NM-2B) in a nucleotide-free state and at a resolution of 2.8A. The structure shows the motor domain with an open active site and the large cleft that divides the 50kDa domain in a closed state. Compared to other rigor-like myosin motor domain structures, our structure shows subtle but significant conformational changes in regions important for actin binding and mechanochemical coupling. Moreover, our crystal structure helps to rationalize the impact of myosin, heavy chain 9 (MYH9)-related disease mutations Arg709Cys and Arg709His on the kinetic and functional properties of NM-2B and of the closely related non-muscle myosin 2A (NM-2A).
Crystal structure of the rigor-like human non-muscle myosin-2 motor domain.,Munnich S, Pathan-Chhatbar S, Manstein DJ FEBS Lett. 2014 Dec 20;588(24):4754-60. doi: 10.1016/j.febslet.2014.11.007. Epub , 2014 Nov 15. PMID:25451231[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Betapudi V. Myosin II motor proteins with different functions determine the fate of lamellipodia extension during cell spreading. PLoS One. 2010 Jan 5;5(1):e8560. doi: 10.1371/journal.pone.0008560. PMID:20052411 doi:http://dx.doi.org/10.1371/journal.pone.0008560
- ↑ Cai L, Fritz D, Stefanovic L, Stefanovic B. Nonmuscle myosin-dependent synthesis of type I collagen. J Mol Biol. 2010 Aug 27;401(4):564-78. doi: 10.1016/j.jmb.2010.06.057. Epub 2010 , Jul 13. PMID:20603131 doi:http://dx.doi.org/10.1016/j.jmb.2010.06.057
- ↑ Munnich S, Pathan-Chhatbar S, Manstein DJ. Crystal structure of the rigor-like human non-muscle myosin-2 motor domain. FEBS Lett. 2014 Dec 20;588(24):4754-60. doi: 10.1016/j.febslet.2014.11.007. Epub , 2014 Nov 15. PMID:25451231 doi:http://dx.doi.org/10.1016/j.febslet.2014.11.007
