1ojt
From Proteopedia
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| , resolution 2.75Å | |||||||
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| Ligands: | |||||||
| Gene: | M-6 OBTAINED FROM A GENOMIC (Neisseria meningitidis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF DIHYDROLIPOAMIDE DEHYDROGENASE
Overview
The protein p64k from the surface of the Neisseria meningitidis bacteria has been characterized as a two-domain protein. It contains a dihydrolipoamide dehydrogenase domain of 482 residues, involving a FAD prosthetic group as a cofactor, and a smaller lipoic acid binding domain of 86 residues. The two domains are joined by a flexible segment rich in alanine and proline residues. The structure of the dihydrolipoamide dehydrogenase domain was determined by X-ray diffraction. It was solved by a combination of molecular replacement and multiple isomorphous replacement techniques and refined to 2.7 A resolution. In the crystal, the recombinant p64k mimics the functional homo-dimer by using one of the crystallographic 2-fold axes. The reactive disulphide bridge Cys161-Cys166 is in the oxidised state and the FAD is bound in an extended conformation. This main domain contains the major antigenic determinant of the protein, an extended loop of 32 residues at the surface of the protein. A mis-attribution at residue 553 in the sequence has been detected by inspection of electron density maps and the geometry. However, when compared to the other dihydrolipoamide dehydrogenases, there are some significant differences: (1) an unusual number of cis-proline residues and (2) a new motif built around a 2-fold axis by the sulphur atoms of residues Met558, Cys560 and their symmetry related equivalents.
About this Structure
1OJT is a Single protein structure of sequence from Neisseria meningitidis. Full crystallographic information is available from OCA.
Reference
Molecular structure of the lipoamide dehydrogenase domain of a surface antigen from Neisseria meningitidis., Li de la Sierra I, Pernot L, Prange T, Saludjian P, Schiltz M, Fourme R, Padron G, J Mol Biol. 1997 May 30;269(1):129-41. PMID:9193005
Page seeded by OCA on Thu Mar 20 13:11:53 2008
