1ovu
From Proteopedia
| |||||||
| , resolution 3.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF FOUR-HELIX BUNDLE MODEL di-Co(II)-DF1-L13A (form I)
Overview
Diffraction data from two non-isomorphous crystals (forms 1 and 2) of an artificial protein with a four-helix bundle motif, di-Co(II)-DF1-L13A, have been collected using synchrotron radiation. The phase of form 1 has been assigned using the group and minimal non-isomorphic supergroup relation between the space group of the previously determined di-Mn(II)-DF1-L13G structure and the space group of this form. This unconventional method of solving the phase problem has also been tested with form 2 using a reverse relation. The structure of the latter form has been solved using the group and maximal non-isomorphic subgroup relation with the space group of form 2 of the analogous dimanganese protein. This application has shown that this phasing method can be used for solving the protein structures of polymorphic crystals as an alternative to the molecular-replacement method.
About this Structure
1OVU is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Phasing protein structures using the group-subgroup relation., Di Costanzo L, Forneris F, Geremia S, Randaccio L, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1435-9. Epub 2003, Jul 23. PMID:12876346
Page seeded by OCA on Thu Mar 20 13:16:20 2008
