Topoisomerase

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Contents 1 Function 2 Relevance 3 GO Annotations 4 3D Structures of Topoisomerase

Function

Topoisomerase (TOP) winds and unwinds DNA double helix in order to enable DNA replication. The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.

• TOPI cuts one strand of the double helix. It has 3 subclasses: TOPIA which shares its mechanism with TOPII and TOPIB which uses rotary mechanism and includes topo I and topo III. TOPIC called TOPV shares its mechanism with TOPIB but is structurally unique. See also Variola Topoisomerase 1B and Smallpox (Variola Virus) - Topoisomerase 1B.
  
• TOPII cuts both strands of the double helix. Its subclasses are TOPIIA including topo II and TOPIIB which includes topo VI.
  
• TOPIII is a potent decatenase.
  
• TOPIV is a type of TOPII, contains an ATP-binding domain ParE and a catalytic domain ParC.
  

Relevance

TOP!V inhibitor Ciprofloxacin is used as an antibacterial antibiotic.

GO Annotations

Database	ID	Symbol	Qualifier	GO Identifier	GO Term Name	Aspect	Evidence	Reference	With	Taxon	Date	Assigned by	Product Form ID
UniProtKB	P14294	topB		GO:0006259	DNA metabolic process	P	IEA	InterPro2GO	InterPro:IPR006154	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0006265	DNA topological change	P	IEA	InterPro2GO	InterPro:IPR000380	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0006265	DNA topological change	P	IEA	InterPro2GO	InterPro:IPR003601	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0006265	DNA topological change	P	IEA	InterPro2GO	InterPro:IPR003602	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0006265	DNA topological change	P	IEA	InterPro2GO	InterPro:IPR005738	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0006265	DNA topological change	P	IEA	InterPro2GO	InterPro:IPR013497	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0006265	DNA topological change	P	IEA	InterPro2GO	InterPro:IPR013824	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0006265	DNA topological change	P	IEA	InterPro2GO	InterPro:IPR013825	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0006268	DNA unwinding involved in replication	P	IEA	InterPro2GO	InterPro:IPR013824	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0006268	DNA unwinding involved in replication	P	IEA	InterPro2GO	InterPro:IPR013825	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0000166	nucleotide binding	F	IEA	Swiss-Prot Keywords2GO	SP_KW:KW-0547	83333	20101127	UniProtKB
UniProtKB	P14294	topB		GO:0003676	nucleic acid binding	F	IEA	InterPro2GO	InterPro:IPR006154	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0003677	DNA binding	F	IEA	InterPro2GO	InterPro:IPR000380	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0003677	DNA binding	F	IEA	InterPro2GO	InterPro:IPR003601	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0003677	DNA binding	F	IEA	InterPro2GO	InterPro:IPR003602	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0003677	DNA binding	F	IEA	InterPro2GO	InterPro:IPR005738	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0003677	DNA binding	F	IEA	InterPro2GO	InterPro:IPR013497	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0003677	DNA binding	F	IEA	InterPro2GO	InterPro:IPR013824	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0003677	DNA binding	F	IEA	InterPro2GO	InterPro:IPR013825	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0003677	DNA binding	F	IEA	Swiss-Prot Keywords2GO	SP_KW:KW-0238	83333	20101127	UniProtKB
UniProtKB	P14294	topB		GO:0003916	DNA topoisomerase activity	F	IEA	InterPro2GO	InterPro:IPR003601	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0003916	DNA topoisomerase activity	F	IEA	InterPro2GO	InterPro:IPR003602	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0003916	DNA topoisomerase activity	F	IEA	InterPro2GO	InterPro:IPR005738	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0003916	DNA topoisomerase activity	F	IEA	InterPro2GO	InterPro:IPR013497	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0003916	DNA topoisomerase activity	F	IEA	InterPro2GO	InterPro:IPR013824	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0003916	DNA topoisomerase activity	F	IEA	InterPro2GO	InterPro:IPR013825	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0003916	DNA topoisomerase activity	F	IEA	Swiss-Prot Keywords2GO	SP_KW:KW-0799	83333	20101127	UniProtKB
UniProtKB	P14294	topB		GO:0003917	DNA topoisomerase type I activity	F	IDA	PMID:6326814		83333	20100621	EcoliWiki
UniProtKB	P14294	topB		GO:0003917	DNA topoisomerase type I activity	F	IEA	InterPro2GO	InterPro:IPR000380	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0003917	DNA topoisomerase type I activity	F	IEA	EC2GO	EC:5.99.1.2	83333	20100703	UniProtKB
UniProtKB	P14294	topB		GO:0005515	protein binding	F	IPI	PMID:15690043	frlR (FRLR_ECOLI)	83333	20101127	IntAct
UniProtKB	P14294	topB		GO:0005524	ATP binding	F	IEA	Swiss-Prot Keywords2GO	SP_KW:KW-0067	83333	20101127	UniProtKB
UniProtKB	P14294	topB		GO:0016853	isomerase activity	F	IEA	Swiss-Prot Keywords2GO	SP_KW:KW-0413	83333	20101127	UniProtKB
UniProtKB	P14294	topB		GO:0005694	chromosome	C	IEA	InterPro2GO	InterPro:IPR000380	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0005694	chromosome	C	IEA	InterPro2GO	InterPro:IPR003601	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0005694	chromosome	C	IEA	InterPro2GO	InterPro:IPR003602	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0005694	chromosome	C	IEA	InterPro2GO	InterPro:IPR005738	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0005694	chromosome	C	IEA	InterPro2GO	InterPro:IPR013497	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0005694	chromosome	C	IEA	InterPro2GO	InterPro:IPR013824	83333	20101127	InterPro
UniProtKB	P14294	topB		GO:0005694	chromosome	C	IEA	InterPro2GO	InterPro:IPR013825	83333	20101127	InterPro

Copied from [1] Last updated Dec 2 2010

3D Structures of Topoisomerase

Updated on 29-February-2016

Created with the participation of Sonny Zhao.