Structural highlights
4a4a is a 1 chain structure with sequence from Clostridium perfringens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , , , , |
| Related: | 2vcc, 2vc9, 4a43, 4a44, 4a41, 4a3z, 2vcb, 4a45, 2vca |
| Activity: | Alpha-N-acetylglucosaminidase, with EC number 3.2.1.50 |
| Resources: | FirstGlance, OCA, RCSB, PDBsum |
Publication Abstract from PubMed
CpGH89 is a family 89 glycoside hydrolase with exo-alpha-D-N-acetylglucosaminidase activity that is produced by the human and animal pathogen Clostridium perfringens. This enzyme is active on the alpha-D-GlcpNAc-(1 --> 4)-D-Galp motif that is displayed on the class III mucins within the gastric mucosa. Other members of this enzyme family, such as human NAGLU, are active on heparan. A truncated version of CpGH89 was rendered inactive through the mutation of two key catalytic residues, the protein crystallized and its structure determined in complex with alpha-D-GlcpNAc-(1 --> 4)-D-Galp to reveal the molecular details of how this unique disaccharide is recognized by CpGH89. An analysis of this substrate complex not only provides insight into how this enzyme selects for its mucin-presented substrate but also advances our understanding of how its clinically relevant mammalian counterparts are specific for heparan.
Structural analysis of a bacterial exo-alpha-D-N-acetylglucosaminidase in complex with an unusual disaccharide found in class III mucin.,Ficko-Blean E, Boraston AB Glycobiology. 2012 May;22(5):590-5. doi: 10.1093/glycob/cwr165. Epub 2011 Nov 16. PMID:22090394[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ficko-Blean E, Boraston AB. Structural analysis of a bacterial exo-alpha-D-N-acetylglucosaminidase in complex with an unusual disaccharide found in class III mucin. Glycobiology. 2012 May;22(5):590-5. doi: 10.1093/glycob/cwr165. Epub 2011 Nov 16. PMID:22090394 doi:10.1093/glycob/cwr165
