Function
2-isopropylmalate synthase (LeuA) or α-isopropylmalate synthase catalyzes the reversible conversion of 3-methyl-2-oxobutanoate and acetyl-CoA to 2-isopropylmalate. LeuA participates in the biosynthesis of leucine and pyruvate metabolism[1].
Structural highlights
LeuA structure is composed of 2 major domains - the catalytic N-terminal and the regulatory C-terminal. The 2 domains are separated by subdomains I and II and a short flexible hinge region between the 2 subdomains. The catalytic domain binds the substrate and the Zn+2 ion[2].
