1qz7
From Proteopedia
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| , resolution 2.20Å | |||||||
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| Gene: | CTNNB1 (Homo sapiens), AXIN (Xenopus laevis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Beta-catenin binding domain of Axin in complex with beta-catenin
Contents |
Overview
The "beta-catenin destruction complex" is central to canonical Wnt/beta-catenin signaling. The scaffolding protein Axin and the tumor suppressor adenomatous polyposis coli protein (APC) are critical components of this complex, required for rapid beta-catenin turnover. We determined the crystal structure of a complex between beta-catenin and the beta-catenin-binding domain of Axin (Axin-CBD). The Axin-CBD forms a helix that occupies the groove formed by the third and fourth armadillo repeats of beta-catenin and thus precludes the simultaneous binding of other beta-catenin partners in this region. Our biochemical studies demonstrate that, when phosphorylated, the 20-amino acid repeat region of APC competes with Axin for binding to beta-catenin. We propose that a key function of APC in the beta-catenin destruction complex is to remove phosphorylated beta-catenin product from the active site.
Disease
Known diseases associated with this structure: Colorectal cancer OMIM:[116806], Hepatoblastoma OMIM:[116806], Hepatocellular carcinoma OMIM:[116806], Ovarian carcinoma, endometrioid type OMIM:[116806], Pilomatricoma OMIM:[116806]
About this Structure
1QZ7 is a Protein complex structure of sequences from Homo sapiens and Xenopus laevis. Full crystallographic information is available from OCA.
Reference
Crystal structure of a beta-catenin/axin complex suggests a mechanism for the beta-catenin destruction complex., Xing Y, Clements WK, Kimelman D, Xu W, Genes Dev. 2003 Nov 15;17(22):2753-64. Epub 2003 Nov 4. PMID:14600025
Page seeded by OCA on Thu Mar 20 13:44:42 2008
