1sjm
From Proteopedia
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| , resolution 1.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , and | ||||||
| Gene: | NIRK, NIR (Alcaligenes faecalis) | ||||||
| Activity: | Nitrite reductase (NO-forming), with EC number 1.7.2.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Nitrite bound copper containing nitrite reductase
Overview
A copper-nitrosyl intermediate forms during the catalytic cycle of nitrite reductase, the enzyme that mediates the committed step in bacterial denitrification. The crystal structure of a type 2 copper-nitrosyl complex of nitrite reductase reveals an unprecedented side-on binding mode in which the nitrogen and oxygen atoms are nearly equidistant from the copper cofactor. Comparison of this structure with a refined nitrite-bound crystal structure explains how coordination can change between copper-oxygen and copper-nitrogen during catalysis. The side-on copper-nitrosyl in nitrite reductase expands the possibilities for nitric oxide interactions in copper proteins such as superoxide dismutase and prions.
About this Structure
1SJM is a Single protein structure of sequence from Alcaligenes faecalis. Full crystallographic information is available from OCA.
Reference
Side-on copper-nitrosyl coordination by nitrite reductase., Tocheva EI, Rosell FI, Mauk AG, Murphy ME, Science. 2004 May 7;304(5672):867-70. PMID:15131305
Page seeded by OCA on Thu Mar 20 14:05:48 2008
Categories: Alcaligenes faecalis | Nitrite reductase (NO-forming) | Single protein | Mauk, A G. | Murphy, M E.P. | Rosell, F I. | Tocheva, E I. | ACT | CU | NO2 | TRS | Copper | Nitrite
