1gwl
From Proteopedia
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CARBOHYDRATE BINDING MODULE FAMILY29 COMPLEXED WITH MANNOHEXAOSE
Overview
Carbohydrate-protein recognition is central to many biological processes., Enzymes that act on polysaccharide substrates frequently contain, noncatalytic domains, "carbohydrate-binding modules" (CBMs), that target, the enzyme to the appropriate substrate. CBMs that recognize specific, plant structural polysaccharides are often able to accommodate both the, variable backbone and the side-chain decorations of heterogeneous ligands., "CBM29" modules, derived from a noncatalytic component of the Piromyces, equi cellulase/hemicellulase complex, provide an example of this selective, yet flexible recognition. They discriminate strongly against some, polysaccharides while remaining relatively promiscuous toward both, beta-1,4-linked manno- and cello-oligosaccharides. This feature may, reflect preferential, but flexible, targeting toward glucomannans in the, plant cell wall. The three-dimensional structure of CBM29-2 and its, complexes with cello- and mannohexaose reveal a beta-jelly-roll topology, with an extended binding groove on the concave surface. The orientation of, the aromatic residues complements the conformation of the target sugar, polymer while accommodation of both manno- and gluco-configured oligo- and, polysaccharides is conferred by virtue of the plasticity of the direct, interactions from their axial and equatorial 2-hydroxyls, respectively., Such flexible ligand recognition targets the anaerobic fungal complex to a, range of different components in the plant cell wall and thus plays a, pivotal role in the highly efficient degradation of this composite, structure by the microbial eukaryote.
About this Structure
1GWL is a Single protein structure of sequence from Piromyces equi. Structure known Active Site: BM1. Full crystallographic information is available from OCA.
Reference
Promiscuity in ligand-binding: The three-dimensional structure of a Piromyces carbohydrate-binding module, CBM29-2, in complex with cello- and mannohexaose., Charnock SJ, Bolam DN, Nurizzo D, Szabo L, McKie VA, Gilbert HJ, Davies GJ, Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14077-82. Epub 2002 Oct 21. PMID:12391332
Page seeded by OCA on Mon Nov 5 12:36:03 2007
