1sxj
From Proteopedia
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| , resolution 2.85Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | RFC1, CDC44, YOR217W, YOR50-7 (Saccharomyces cerevisiae), RFC4, YOL094C, O0923 (Saccharomyces cerevisiae), RFC3, YNL290W, N0533 (Saccharomyces cerevisiae), RFC2, YJR068W, J1808 (Saccharomyces cerevisiae), RFC5, YBR087W, YBR0810 (Saccharomyces cerevisiae), POL30, YBR088C, YBR0811 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Eukaryotic Clamp Loader (Replication Factor C, RFC) Bound to the DNA Sliding Clamp (Proliferating Cell Nuclear Antigen, PCNA)
Overview
Sliding clamps are ring-shaped proteins that encircle DNA and confer high processivity on DNA polymerases. Here we report the crystal structure of the five-protein clamp loader complex (replication factor-C, RFC) of the yeast Saccharomyces cerevisiae, bound to the sliding clamp (proliferating cell nuclear antigen, PCNA). Tight interfacial coordination of the ATP analogue ATP-gammaS by RFC results in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring. Placement of a model for primed DNA within the central hole of PCNA reveals a striking correspondence between the RFC spiral and the grooves of the DNA double helix. This model, in which the clamp loader complex locks onto primed DNA in a screw-cap-like arrangement, provides a simple explanation for the process by which the engagement of primer-template junctions by the RFC:PCNA complex results in ATP hydrolysis and release of the sliding clamp on DNA.
About this Structure
1SXJ is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex., Bowman GD, O'Donnell M, Kuriyan J, Nature. 2004 Jun 17;429(6993):724-30. PMID:15201901
Page seeded by OCA on Sun Mar 23 13:40:03 2008
