1uup
From Proteopedia
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CRYSTAL STRUCTURE OF A DIMERIC FORM OF STREPTOCOCCAL PYROGENIC EXOTOXIN A (SPEA1).
Overview
Streptococcal pyrogenic exotoxin A (SpeA1) is a bacterial superantigen associated with scarlet fever and streptococcal toxic shock syndrome (STSS). SpeA1 is found in both monomeric and dimeric forms, and previous work suggested that the dimer results from an intermolecular disulfide bond between the cysteines at positions 90 of each monomer. Here, we present the crystal structure of the dimeric form of SpeA1. The toxin crystallizes in the orthorhombic space group P212121, with two dimers in the crystallographic asymmetric unit. The final structure has a crystallographic R-factor of 21.52% for 7248 protein atoms, 136 water molecules, and 4 zinc atoms (one zinc atom per molecule). The implications of SpeA1 dimer on MHC class II and T-cell receptor recognition are discussed.
About this Structure
1UUP is a Single protein structure of sequence from Streptococcus pyogenes. Full crystallographic information is available from OCA.
Reference
Crystal structure of a dimeric form of streptococcal pyrogenic exotoxin A (SpeA1)., Baker MD, Gendlina I, Collins CM, Acharya KR, Protein Sci. 2004 Sep;13(9):2285-90. Epub 2004 Aug 4. PMID:15295110
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