1xt6

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Image:1xt6.gif


PDB ID 1xt6

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, resolution 1.80Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



S35C Flavodoxin Mutant in the semiquinone state


Overview

The crystallographic structure of an engineered flavodoxin mutant from Desulfovibrio vulgaris has been analysed. Site-directed mutagenesis was used to substitute serine 35 with a cysteine to provide a possible covalent linkage. The crystal structure of the semiquinone form of this mutant is similar to the corresponding oxidation state of the wild-type flavodoxin. Analysis of the structural changes reveals the interaction between N(5)H of the flavin and the carbonyl O atom of Gly61 to be critical for modulation of the electrochemical properties of the protein.

About this Structure

1XT6 is a Single protein structure of sequence from Desulfovibrio vulgaris. Full crystallographic information is available from OCA.

Reference

Structure of S35C flavodoxin mutant from Desulfovibrio vulgaris in the semiquinone state., Artali R, Marchini N, Meneghetti F, Cavazzini D, Cassetta A, Sassone C, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):481-4. Epub 2005, Mar 24. PMID:15805604

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