Tubulin tyrosine ligase

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spinqualitylabelsall models Tubulin tyrosine ligase (cyan) complex with α-tubulin (grey and pink), β-tubulin (green and yellow), stathmin (magenta), MES, GTP, Ca+2, Cl- and Mg+2 ions (PDB code 4iij) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Function Tubulin tyrosine ligase (TTL) adds tyrosine in an ATP-dependent reaction to the C-terminal of a detyrosinated a-tubulin. The detyrosination of α-tubulin is a posttranslational modification which exposes glutamate in the new tubulin C-terminal. The detyrosinated α-tubulin forms Glu-microtubules[1]. The complex of TTL and tubulin contains Stathmin (STM) - another tubulin-binding protein[2]. Disease Mutated TTL causes morphogenic abnormalities and cancer. There is a loss of TTL activity during tumor growth[3]. Structural highlights . TTL structure is composed of . via 3 loops and one helix terminal[4].

3D Structures of tubulin tyrosine ligase

Updated on 12-October-2017

4o2a, 5fnv, 5ca1 – cTTL + rSTM + tubulin + GTP + GDP + ADP + inhibitor
4i4t – cTTL + rSTM + tubulin + GTP + GDP + zampanolide
4i50 – cTTL + rSTM + tubulin + GTP + GDP + epothilone
4o4l, 4tuy, 4tv8, 4yj2, 4yj3 – cTTL + rSTM + tubulin + GTP + GDP + anti-cancer drugs
4o2b – cTTL + rSTM + tubulin + GTP + GDP + ATP derivative + anti-gout drug
4o4h, 4o4j, 4o4i – cTTL + rSTM + tubulin + GTP + GDP + ATP derivative + anti-cancer drug

References

1. ↑ Idriss HT. Phosphorylation of tubulin tyrosine ligase: a potential mechanism for regulation of alpha-tubulin tyrosination. Cell Motil Cytoskeleton. 2000 May;46(1):1-5. PMID:10842328 doi:<1::AID-CM1>3.0.CO;2-6 http://dx.doi.org/10.1002/(SICI)1097-0169(200005)46:1<1::AID-CM1>3.0.CO;2-6
2. ↑ Szyk A, Piszczek G, Roll-Mecak A. Tubulin tyrosine ligase and stathmin compete for tubulin binding in vitro. J Mol Biol. 2013 Jul 24;425(14):2412-4. doi: 10.1016/j.jmb.2013.04.017. Epub 2013, Apr 25. PMID:23624152 doi:http://dx.doi.org/10.1016/j.jmb.2013.04.017
3. ↑ Lafanechere L, Courtay-Cahen C, Kawakami T, Jacrot M, Rudiger M, Wehland J, Job D, Margolis RL. Suppression of tubulin tyrosine ligase during tumor growth. J Cell Sci. 1998 Jan;111 ( Pt 2):171-81. PMID:9405300
4. ↑ Prota AE, Magiera MM, Kuijpers M, Bargsten K, Frey D, Wieser M, Jaussi R, Hoogenraad CC, Kammerer RA, Janke C, Steinmetz MO. Structural basis of tubulin tyrosination by tubulin tyrosine ligase. J Cell Biol. 2013 Feb 4;200(3):259-70. doi: 10.1083/jcb.201211017. Epub 2013 Jan , 28. PMID:23358242 doi:http://dx.doi.org/10.1083/jcb.201211017