Structural highlights
Function
[CUER_ECOLI] Regulates the transcription of the copA and cueO genes. It detects cytoplasmic copper stress and activates transcription in response to increasing copper concentrations.[1] [2]
Publication Abstract from PubMed
Many transcriptional activators act at a distance from core promoter elements and work by recruiting RNA polymerase through protein-protein interactions. We show here how the prokaryotic regulatory protein CueR both represses and activates transcription by differentially modulating local DNA structure within the promoter. Structural studies reveal that the repressor state slightly bends the promoter DNA, precluding optimal RNA polymerase-promoter recognition. Upon binding a metal ion in the allosteric site, CueR switches into an activator conformation. It maintains all protein-DNA contacts but introduces torsional stresses that kink and undertwist the promoter, stabilizing an A-form DNA-like conformation. These factors switch on and off transcription by exerting dynamic control of DNA stereochemistry, reshaping the core promoter and making it a better or worse substrate for polymerase.
TRANSCRIPTION. Allosteric transcriptional regulation via changes in the overall topology of the core promoter.,Philips SJ, Canalizo-Hernandez M, Yildirim I, Schatz GC, Mondragon A, O'Halloran TV Science. 2015 Aug 21;349(6250):877-81. doi: 10.1126/science.aaa9809. PMID:26293965[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Outten FW, Outten CE, Hale J, O'Halloran TV. Transcriptional activation of an Escherichia coli copper efflux regulon by the chromosomal MerR homologue, cueR. J Biol Chem. 2000 Oct 6;275(40):31024-9. PMID:10915804 doi:http://dx.doi.org/10.1074/jbc.M006508200
- ↑ Outten FW, Huffman DL, Hale JA, O'Halloran TV. The independent cue and cus systems confer copper tolerance during aerobic and anaerobic growth in Escherichia coli. J Biol Chem. 2001 Aug 17;276(33):30670-7. Epub 2001 Jun 8. PMID:11399769 doi:http://dx.doi.org/10.1074/jbc.M104122200
- ↑ Philips SJ, Canalizo-Hernandez M, Yildirim I, Schatz GC, Mondragon A, O'Halloran TV. TRANSCRIPTION. Allosteric transcriptional regulation via changes in the overall topology of the core promoter. Science. 2015 Aug 21;349(6250):877-81. doi: 10.1126/science.aaa9809. PMID:26293965 doi:http://dx.doi.org/10.1126/science.aaa9809
