Structural highlights
Function
[ERF1_HUMAN] Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.[1] [RL12_HUMAN] Binds directly to 26S ribosomal RNA (By similarity).
Publication Abstract from PubMed
In contrast to bacteria that have two release factors, RF1 and RF2, eukaryotes only possess one unrelated release factor eRF1, which recognizes all three stop codons of the mRNA and hydrolyses the peptidyl-tRNA bond. While the molecular basis for bacterial termination has been elucidated, high-resolution structures of eukaryotic termination complexes have been lacking. Here we present a 3.8 A structure of a human translation termination complex with eRF1 decoding a UAA(A) stop codon. The complex was formed using the human cytomegalovirus (hCMV) stalling peptide, which perturbs the peptidyltransferase center (PTC) to silence the hydrolysis activity of eRF1. Moreover, unlike sense codons or bacterial stop codons, the UAA stop codon adopts a U-turn-like conformation within a pocket formed by eRF1 and the ribosome. Inducing the U-turn conformation for stop codon recognition rationalizes how decoding by eRF1 includes monitoring geometry in order to discriminate against sense codons.
Structure of a human translation termination complex.,Matheisl S, Berninghausen O, Becker T, Beckmann R Nucleic Acids Res. 2015 Oct 15;43(18):8615-26. doi: 10.1093/nar/gkv909. Epub 2015, Sep 17. PMID:26384426[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Frolova L, Le Goff X, Rasmussen HH, Cheperegin S, Drugeon G, Kress M, Arman I, Haenni AL, Celis JE, Philippe M, et al.. A highly conserved eukaryotic protein family possessing properties of polypeptide chain release factor. Nature. 1994 Dec 15;372(6507):701-3. PMID:7990965 doi:http://dx.doi.org/10.1038/372701a0
- ↑ Matheisl S, Berninghausen O, Becker T, Beckmann R. Structure of a human translation termination complex. Nucleic Acids Res. 2015 Oct 15;43(18):8615-26. doi: 10.1093/nar/gkv909. Epub 2015, Sep 17. PMID:26384426 doi:http://dx.doi.org/10.1093/nar/gkv909
