2d6f
From Proteopedia
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Crystal structure of Glu-tRNA(Gln) amidotransferase in the complex with tRNA(Gln)
Overview
Glutaminyl-transfer RNA (Gln-tRNA(Gln)) in archaea is synthesized in a pretranslational amidation of misacylated Glu-tRNA(Gln) by the heterodimeric Glu-tRNA(Gln) amidotransferase GatDE. Here we report the crystal structure of the Methanothermobacter thermautotrophicus GatDE complexed to tRNA(Gln) at 3.15 angstroms resolution. Biochemical analysis of GatDE and of tRNA(Gln) mutants characterized the catalytic centers for the enzyme's three reactions (glutaminase, kinase, and amidotransferase activity). A 40 angstrom-long channel for ammonia transport connects the active sites in GatD and GatE. tRNA(Gln) recognition by indirect readout based on shape complementarity of the D loop suggests an early anticodon-independent RNA-based mechanism for adding glutamine to the genetic code.
About this Structure
2D6F is a Protein complex structure of sequences from Methanothermobacter thermautotrophicus. Full crystallographic information is available from OCA.
Reference
Structural basis of RNA-dependent recruitment of glutamine to the genetic code., Oshikane H, Sheppard K, Fukai S, Nakamura Y, Ishitani R, Numata T, Sherrer RL, Feng L, Schmitt E, Panvert M, Blanquet S, Mechulam Y, Soll D, Nureki O, Science. 2006 Jun 30;312(5782):1950-4. PMID:16809540
Page seeded by OCA on Thu Mar 20 16:23:50 2008
