Green Florescent protein is a protein that converts the blue chemiluminescent of aequorin in the jellyfish into green fluorescent light. Because of it's green fluorescence, GFP can be utilized as a marker protein when incorporated into a variety of biological molecules and systems. This is useful for gene cloning, because it allows for researchers to determine from its use as a marker if their gene has been successfully incorporated into the cloning vector and replicated in their organism of choice. For example, if the gene coding for insulin was attached with the GFP, and the inserted into a cloning vector, the vector can then be electroporated into a fast growing organism like E.coli. After this, scientists can view if the gene was successfully incorporated based on if the ecoli is fluorescent or not.
Regarding the structure, the protein is shaped like a barrel, which is from the folding of the in the secondary structure. In addition, can be found running through the center of the barrel.
