2fqn
From Proteopedia
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| , resolution 2.300Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site
Overview
The decoding A site of the small ribosomal subunit is an RNA molecular switch, which monitors codon-anticodon interactions to guarantee translation fidelity. We have solved the crystal structure of an RNA fragment containing two Homo sapiens cytoplasmic A sites. Each of the two A sites presents a different conformational state. In one state, adenines A1492 and A1493 are fully bulged-out with C1409 forming a wobble-like pair to A1491. In the second state, adenines A1492 and A1493 form non-Watson-Crick pairs with C1409 and G1408, respectively while A1491 bulges out. The first state of the eukaryotic A site is, thus, basically the same as in the bacterial A site with bulging A1492 and A1493. It is the state used for recognition of the codon/anticodon complex. On the contrary, the second state of the H.sapiens cytoplasmic A site is drastically different from any of those observed for the bacterial A site without bulging A1492 and A1493.
About this Structure
2FQN is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Two conformational states in the crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site., Kondo J, Urzhumtsev A, Westhof E, Nucleic Acids Res. 2006 Feb 1;34(2):676-85. Print 2006. PMID:16452297
Page seeded by OCA on Thu Mar 20 16:55:40 2008
Categories: Protein complex | Kondo, J. | Urzhumtsev, A. | Westhof, E. | MG | NCO | A site | Decoding site | Homo sapiens cytoplasm | Rna duplex | Rrna
