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2ggm

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Revision as of 15:04, 20 March 2008 by OCA (Talk | contribs)
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PDB ID 2ggm

Drag the structure with the mouse to rotate
, resolution 2.35Å
Ligands:
Gene: CETN2, CALT, CEN2 (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



Human centrin 2 xeroderma pigmentosum group C protein complex


Contents

Overview

Human centrin-2 plays a key role in centrosome function and stimulates nucleotide excision repair by binding to the xeroderma pigmentosum group C protein. To determine the structure of human centrin-2 and to develop an understanding of molecular interactions between centrin and xeroderma pigmentosum group C protein, we characterized the crystal structure of calcium-loaded full-length centrin-2 complexed with a xeroderma pigmentosum group C peptide. Our structure shows that the carboxyl-terminal domain of centrin-2 binds this peptide and two calcium atoms, whereas the amino-terminal lobe is in a closed conformation positioned distantly by an ordered alpha-helical linker. A stretch of the amino-terminal domain unique to centrins appears disordered. Two xeroderma pigmentosum group C peptides both bound to centrin-2 also interact to form an alpha-helical coiled-coil. The interface between centrin-2 and each peptide is predominantly nonpolar, and key hydrophobic residues of XPC have been identified that lead us to propose a novel binding motif for centrin.

Disease

Known diseases associated with this structure: Xeroderma pigmentosum, group C OMIM:[278720]

About this Structure

2GGM is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The structure of the human centrin 2-xeroderma pigmentosum group C protein complex., Thompson JR, Ryan ZC, Salisbury JL, Kumar R, J Biol Chem. 2006 Jul 7;281(27):18746-52. Epub 2006 Apr 20. PMID:16627479

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