2gjw
From Proteopedia
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| , resolution 2.85Å | |||||||
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| Gene: | endA (Archaeoglobus fulgidus) | ||||||
| Activity: | tRNA-intron endonuclease, with EC number 3.1.27.9 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RNA Recognition and Cleavage by an Splicing Endonuclease
Overview
The RNA splicing endonuclease cleaves two phosphodiester bonds within folded precursor RNAs during intron removal, producing the functional RNAs required for protein synthesis. Here we describe at a resolution of 2.85 angstroms the structure of a splicing endonuclease from Archaeglobus fulgidus bound with a bulge-helix-bulge RNA containing a noncleaved and a cleaved splice site. The endonuclease dimer cooperatively recognized a flipped-out bulge base and stabilizes sharply bent bulge backbones that are poised for an in-line RNA cleavage reaction. Cooperativity arises because an arginine pair from one catalytic domain sandwiches a nucleobase within the bulge cleaved by the other catalytic domain.
About this Structure
2GJW is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.
Reference
RNA recognition and cleavage by a splicing endonuclease., Xue S, Calvin K, Li H, Science. 2006 May 12;312(5775):906-10. PMID:16690865
Page seeded by OCA on Thu Mar 20 17:05:35 2008
