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1w9d
From Proteopedia
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S. ALBA MYROSINASE IN COMPLEX WITH S-ETHYL PHENYLACETOTHIOHYDROXIMATE-O-SULFATE
Overview
Myrosinase, a thioglucoside glucohydrolase, is the only enzyme able to, hydrolyse glucosinolates, a unique family of molecules bearing an anomeric, O-sulfated thiohydroximate function. Non-hydrolysable myrosinase, inhibitors have been devised and studied for their biological interaction., Diverse modifications of the O-sulfate moiety did not result in a, significant inhibitory effect, whereas replacing the D-glucopyrano residue, by its carba-analogue allowed inhibition to take place. X-Ray experiments, carried out after soaking allowed for the first time inclusion of a, non-hydrolysable inhibitor inside the enzymatic pocket. Structural tuning, of the aglycon part in its pocket is being used as a guide for the, development of simplified and more potent inhibitors.
About this Structure
1W9D is a Single protein structure of sequence from Sinapis alba with NAG, ZN, SO4, SEH and GOL as ligands. Active as Transferred entry: 3.2.1.147, with EC number 3.2.3.1 Structure known Active Site: AGB. Full crystallographic information is available from OCA.
Reference
The glucosinolate-myrosinase system. New insights into enzyme-substrate interactions by use of simplified inhibitors., Bourderioux A, Lefoix M, Gueyrard D, Tatibouet A, Cottaz S, Arzt S, Burmeister WP, Rollin P, Org Biomol Chem. 2005 May 21;3(10):1872-9. Epub 2005 Apr 14. PMID:15889170
Page seeded by OCA on Mon Nov 5 15:06:36 2007
Categories: Sinapis alba | Single protein | Transferred entry: 3.2.1.147 | Arzt, S. | Bourderioux, A. | Burmeister, W.P. | Cottaz, S. | Gueyrard, D. | Lefoix, M. | Rollin, P. | Tatibouet, A. | GOL | NAG | SEH | SO4 | ZN | Glucotropaeolin | Glusosinolate | Hydrolase | Myrosinase | Thio-glucoside | Thioglucosidase | Thioglycosidase | Thiohydroximate
