Structural highlights
Function
[HNF6_RAT] Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription. The affinity of HNF-6-alpha and HNF-6-beta for DNA differs depending on the target sequence.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Hepatocyte nuclear factor-6 (HNF-6), a liver-enriched transcription factor, controls the development of various tissues, such as the pancreas and liver, and regulates the expression of several hepatic genes. This protein belongs to the ONECUT class of homeodomain proteins and contains a bipartite DNA-binding domain composed of a single cut domain and a characteristic homeodomain. This transcription factor has two distinct modes of DNA binding and transcriptional activation that use different coactivators depending on the target gene. The crystal structure of the bipartite DNA-binding domain of HNF-6alpha complexed with the HNF-6-binding site of the TTR promoter revealed the DNA recognition mechanism of this protein. Comparing our structure with the DNA-free structure of HNF-6 or the structure of Oct-1, we discuss characteristic features associated with DNA binding and the structural basis for the dual mode of action of this protein, and we suggest a strategy for variability of transcriptional activation of the target gene.
DNA recognition mechanism of the ONECUT homeodomain of transcription factor HNF-6.,Iyaguchi D, Yao M, Watanabe N, Nishihira J, Tanaka I Structure. 2007 Jan;15(1):75-83. PMID:17223534[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Iyaguchi D, Yao M, Watanabe N, Nishihira J, Tanaka I. DNA recognition mechanism of the ONECUT homeodomain of transcription factor HNF-6. Structure. 2007 Jan;15(1):75-83. PMID:17223534 doi:10.1016/j.str.2006.11.004
