Structural highlights
Function
[CAMA_PSEPU] The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation.[1] [PUTX_PSEPU] The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
In the camphor monooxygenase system from Pseudomonas putida, the [2Fe-2S]-containing putidaredoxin (Pdx) shuttles electrons between the NADH-dependent putidaredoxin reductase (Pdr) and cytochrome P450(cam). The mechanism of the Pdr.Pdx redox couple has been investigated by a variety of techniques. One of the exceptions is x-ray crystallography as the native partners associate weakly and resist co-crystallization. Here, we present the 2.6-A x-ray structure of a catalytically active complex between Pdr and Pdx C73S/C85S chemically cross-linked via the Lys(409Pdr)-Glu(72Pdx) pair. The 365 A(2) Pdr-Pdx interface is predominantly hydrophobic with one central Arg(310Pdr)-Asp(38Pdx) salt bridge, likely assisting docking and orienting the partners optimally for electron transfer, and a few peripheral hydrogen bonds. A predicted 12-A-long electron transfer route between FAD and [2Fe-2S] includes flavin flanking Trp(330Pdr) and the iron ligand Cys(39Pdx). The x-ray model agrees well with the experimental and theoretical results and suggests that the linked Pdx must undergo complex movements during turnover to accommodate P450(cam), which could limit the Pdx-to-P450(cam) electron transfer reaction.
Crystal structure of the putidaredoxin reductase x putidaredoxin electron transfer complex.,Sevrioukova IF, Poulos TL, Churbanova IY J Biol Chem. 2010 Apr 30;285(18):13616-20. Epub 2010 Feb 23. PMID:20179327[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Sevrioukova IF, Poulos TL. Putidaredoxin reductase, a new function for an old protein. J Biol Chem. 2002 Jul 12;277(28):25831-9. Epub 2002 May 13. PMID:12011076 doi:http://dx.doi.org/10.1074/jbc.M201110200
- ↑ Sevrioukova IF, Poulos TL, Churbanova IY. Crystal structure of the putidaredoxin reductase x putidaredoxin electron transfer complex. J Biol Chem. 2010 Apr 30;285(18):13616-20. Epub 2010 Feb 23. PMID:20179327 doi:10.1074/jbc.M110.104968
