3wi3
From Proteopedia
Crystal Structure of the Sld3/Treslin domain from yeast Sld3
Structural highlights
Function[SLD3_YEAST] Required for loading and maintenance of stable association of CDC45 with chromatin during initiation and elongation of DNA replication. Also involved in temporal regulation of origin firing. Required for the association of PSF1 with origins.[1] [2] [3] Publication Abstract from PubMedThe initiation of eukaryotic chromosomal DNA replication requires the formation of an active replicative helicase at the replication origins of chromosomal DNA. Yeast Sld3 and its metazoan counterpart Treslin are the hub proteins mediating protein associations critical for the helicase formation. Here, we show the crystal structure of the central domain of Sld3 that is conserved in Sld3/Treslin family of proteins. The domain consists of two segments with 12 helices and is sufficient to bind to Cdc45, the essential helicase component. The structure model of the Sld3-Cdc45 complex, which is crucial for the formation of the active helicase, is proposed. Crystal structure of the homology domain of the eukaryotic DNA replication proteins sld3/treslin.,Itou H, Muramatsu S, Shirakihara Y, Araki H Structure. 2014 Sep 2;22(9):1341-7. doi: 10.1016/j.str.2014.07.001. Epub 2014 Aug, 7. PMID:25126958[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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