1sjw

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spinqualitylabelsall models PDB ID 1sjw Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
, resolution 1.35Å
Ligands:
Gene:	SnoaL (Streptomyces nogalater)
Resources:	FirstGlance, OCA, PDBsum, RCSB
Coordinates:	save as pdb, mmCIF, xml

Structure of polyketide cyclase SnoaL

Overview

SnoaL belongs to a family of small polyketide cyclases, which catalyse ring closure steps in the biosynthesis of polyketide antibiotics produced in Streptomyces. Several of these antibiotics are among the most used anti-cancer drugs currently in use. The crystal structure of SnoaL, involved in nogalamycin biosynthesis, with a bound product, has been determined to 1.35 A resolution. The fold of the subunit can be described as a distorted alpha+beta barrel, and the ligand is bound in the hydrophobic interior of the barrel. The 3D structure and site-directed mutagenesis experiments reveal that the mechanism of the intramolecular aldol condensation catalysed by SnoaL is different from that of the classical aldolases, which employ covalent Schiff base formation or a metal ion cofactor. The invariant residue Asp121 acts as an acid/base catalyst during the reaction. Stabilisation of the enol(ate) intermediate is mainly achieved by the delocalisation of the electron pair over the extended pi system of the substrate. These polyketide cyclases thus form of family of enzymes with a unique catalytic strategy for aldol condensation.

About this Structure

1SJW is a Single protein structure of sequence from Streptomyces nogalater. Full crystallographic information is available from OCA.

Reference

Structure of the polyketide cyclase SnoaL reveals a novel mechanism for enzymatic aldol condensation., Sultana A, Kallio P, Jansson A, Wang JS, Niemi J, Mantsala P, Schneider G, EMBO J. 2004 May 5;23(9):1911-21. Epub 2004 Apr 8. PMID:15071504

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