PduCas13b is a class 2 type 4 from Prevotella buccae. As a member of this protein family, it uses a single effector complexed with a CRISPR-RNA to bind and cleave RNA with its twin HEPN domains, activating a general non-specific RNase activity that degrades any nearby transcripts.(1) However, PduCas13b is structurally and mechanically different from the other Cas13s, as it has a unique linear organization and different dynamics in target recognition.(2).
Structure
Though the overall conserved bilobed shape of many Class 2 effectors consisting of a recognition and nuclease lobe is present,(2,3) Cas13b has a unique domain organization even among Cas13s. (Fig.1). A crystal structure of PbuCas13b complexed with a 36-nt direct repeat sequence and a 5-nt spacer at 1.65 Å provides information of the . There are five domains within the effector structure: two HEPN domains (),
cnRNA and Targeting
Application
