2v2k

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spinqualitylabelsall models 2v2k, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

THE CRYSTAL STRUCTURE OF FDXA, A 7FE FERREDOXIN FROM MYCOBACTERIUM SMEGMATIS

Overview

Mycobacterium smegmatis ferredoxin FdxA, which has an orthologue, ferredoxin in Mycobacterium tuberculosis, FdxC, contains both one [3Fe-4S], and one [4Fe-4S] cluster. M. smegmatis FdxA has been shown to be a, preferred ferredoxin substrate of FprA [F. Fischer, D. Raimondi, A., Aliverti, G. Zanetti, Mycobacterium tuberculosis FprA, a novel bacterial, NADPH-ferredoxin reductase, Eur. J. Biochem. 269 (2002) 3005-3013], an, adrenodoxin reductase-like flavoprotein of M. tuberculosis, suggesting, that M. tuberculosis FdxC could be the physiological partner of the enzyme, in providing reducing power to the cytochromes P450. We report here the, crystal structure of FdxA at 1.6A resolution (R(factor) 16.5%, R(free), 20.2%). Besides providing an insight on protein architecture for this, 106-residue ferredoxin, our crystallographic investigation highlights, lability of the [4Fe-4S] center, which is shown to loose a Fe atom during, crystal growth. Due to their high similarity (87% sequence identity), the, structure here reported can be considered a valuable model for M., tuberculosis FdxC, thus representing a step forward in the study of the, complex mycobacterial redox pathways.

About this Structure

2V2K is a Single protein structure of sequence from Mycobacterium smegmatis with ACT and F3S as ligands. Structure known Active Site: AC1. Full crystallographic information is available from OCA.

Reference

The crystal structure of FdxA, a 7Fe ferredoxin from Mycobacterium smegmatis., Ricagno S, de Rosa M, Aliverti A, Zanetti G, Bolognesi M, Biochem Biophys Res Commun. 2007 Aug 17;360(1):97-102. Epub 2007 Jun 12. PMID:17577575

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