4tkr

Publication Abstract from PubMed

Anomalous diffraction signals from typical native macromolecules are very weak, frustrating their use in de novo structure determination. Here, native SAD procedures are described to enhance signal to noise in anomalous diffraction by using multiple crystals in combination with synchrotron X-rays at 6 keV. Increased anomalous signals were obtained at 6 keV compared with 7 keV X-ray energy, which was used for previous native SAD analyses. A feasibility test of multi-crystal-based native SAD phasing was performed at 3.2 A resolution for a known tyrosine protein kinase domain, and real-life applications were made to two novel membrane proteins at about 3.0 A resolution. The three applications collectively serve to validate the robust feasibility of native SAD phasing at lower energy.

Multi-crystal native SAD analysis at 6 keV.,Liu Q, Guo Y, Chang Y, Cai Z, Assur Z, Mancia F, Greene MI, Hendrickson WA Acta Crystallogr D Biol Crystallogr. 2014 Oct 1;70(Pt 10):2544-57. doi:, 10.1107/S1399004714013376. Epub 2014 Sep 30. PMID:25286840^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.